Mapping key functional sites within yeast TFIID.

Claire Leurent; Steven L Sanders; Màté A Demény; Krassimira A Garbett; Christine Ruhlmann; P Anthony Weil; Làszlò Tora; Patrick Schultz

doi:10.1038/sj.emboj.7600111

PubMed · 14765106

Mapping key functional sites within yeast TFIID.

Claire Leurent·Steven L Sanders·Màté A Demény·Krassimira A Garbett·Christine Ruhlmann·P Anthony Weil·Làszlò Tora·Patrick Schultz

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Abstract

The transcription factor TFIID, composed of the TATA box-binding protein (TBP) and 14 TBP-associated factors (TAFs), plays a key role in the regulation of gene expression by RNA polymerase II. The structure of yeast TFIID, as determined by electron microscopy and digital image analysis, is formed by three lobes, labelled A-C, connected by thin linking domains. Immunomapping revealed that TFIID contains two copies of the WD-40 repeat-containing TAF5 and that TAF5 contributes to the linkers since its C- and N-termini were found in different lobes. This property was confirmed by the finding that a recombinant complex containing TAF5 complexed with six histone fold containing TAFs was able to form a trilobed structure. Moreover, the N-terminal domain of TAF1 was mapped in lobe C, whereas the histone acetyltransferase domain resides in lobe A along with TAF7. TBP was found in the linker domain between lobes A and C in a way that the N-terminal 100 residues of TAF1 are spanned over it. The implications of these data with regard to TFIID function are discussed.

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Acetyltransferases Fungal Proteins Histone Acetyltransferases Microscopy, Electron, Transmission Models, Molecular Protein Structure, Quaternary Protein Structure, Tertiary RNA Polymerase II Recombinant Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIID Journal Article Research Support, Non-U.S. Gov't

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Claire Leurent, Steven L Sanders, Màté A Demény, Krassimira A Garbett, Christine Ruhlmann, P Anthony Weil, Làszlò Tora, Patrick Schultz. 2004-02-12. Mapping key functional sites within yeast TFIID.. https://doi.org/10.1038/sj.emboj.7600111

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