A simple, efficient method to create a cDNA library.

W N Hu; W Kopachik; R N Band

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PubMed · 1476737

A simple, efficient method to create a cDNA library.

W N Hu·W Kopachik·R N Band

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Abstract

The simplified "All In One Tube" protocol for constructing a cDNA library combines the advantages of the "Classic method" and the Okayama-Berg method while overcoming some of their drawbacks. In this method, adding adapters, linkers or enzyme digestion steps are no longer necessary after cDNA synthesis, thus making it quicker and especially useful when dealing with small samples of mRNA.

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Animals Base Sequence Cloning, Molecular DNA, Protozoan Evaluation Studies as Topic Gene Library Genetic Techniques Genetic Vectors Molecular Sequence Data Naegleria Journal Article

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W N Hu, W Kopachik, R N Band. 1992. A simple, efficient method to create a cDNA library.. https://pubmed.ncbi.nlm.nih.gov/1476737/

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