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PubMed · 1477116

Three electrophoretic techniques comparison for des-gamma-carboxyprothrombin detection.

M Belle·M Guillaumont·M Leclercq·R Guinet
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Abstract

Des-gamma-carboxyprothrombin (DCP) is a marker that appears in the blood when modifications of vitamin K-dependent proteins carboxylation cycle occur. About 280 human plasma samples of diverse origins were tested by three different electrophoretic techniques for the evaluation of DCP: rocket immunoelectrophoresis (RIE) before and after barium carbonate adsorption, crossed affinoimmunoelectrophoresis (CAIE) and polyacrylamide gel electrophoresis in presence of calcium lactate followed by immunoblotting (PAGE-blot). A good correlation was found between CAIE and PAGE-blot in the CAIE detection limit, but not between RIE and the two other techniques. PAGE-blot was more sensitive than RIE and CAIE and allowed reliable quantification of abnormal prothrombin in plasma.

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AdsorptionAdultAnticoagulantsBariumBiomarkersBlood Protein ElectrophoresisCarbonatesCystic FibrosisElectrophoresis, Polyacrylamide GelEvaluation Studies as TopicHumansImmunoelectrophoresisImmunoelectrophoresis, Two-DimensionalInfant, NewbornLiver DiseasesProtein PrecursorsProthrombinSensitivity and SpecificityVitamin K DeficiencyComparative StudyJournal Article

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M Belle, M Guillaumont, M Leclercq, R Guinet. 1992. Three electrophoretic techniques comparison for des-gamma-carboxyprothrombin detection.. https://pubmed.ncbi.nlm.nih.gov/1477116/

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