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PubMed · 1477660

Simplified template preparation and improved direct sequencing using Taq polymerase.

A K Srivastava·V Montanaro·J Kere
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Abstract

A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.

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DNA-Directed DNA PolymeraseElectrophoresis, Agar GelPolymerase Chain ReactionSequence Analysis, DNATaq PolymeraseTemplates, GeneticJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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A K Srivastava, V Montanaro, J Kere. 1992. Simplified template preparation and improved direct sequencing using Taq polymerase.. https://doi.org/10.1101/gr.1.4.255

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