PubMed · 14802999
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Z I IMRE, A L TERZIN. 1950. [Penicillinase in bacteriological diagnoses].. https://pubmed.ncbi.nlm.nih.gov/14802999/
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The beta-lactamase of Streptomyces UCSM-104 behaves as a penicillinase with the different substrates studied, being more active with benzylpenicillin (KM 2.6 mM) and ampicillin (KM 1.5 mM). On the other hand, it was competitively inhibited by methicillin (Ki 0.035 mM) and cloxacillin (Ki 0.35 mM) using benzylpenicillin as substrate. According to the criterion of Jack and Richmond in relation to the degree of inhibition obtained, the enzyme was found to be resistant to cloxacillin and sensible to methicillin.
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