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PubMed · 14872098

Imaging calcium concentration dynamics in small neuronal compartments.

Ryohei Yasuda·Esther A Nimchinsky·Volker Scheuss·Thomas A Pologruto·Thomas G Oertner·Bernardo L Sabatini·Karel Svoboda
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Abstract

Calcium and its regulation play central roles diverse physiologic processes. Quantification of calcium concentrations ([Ca2+]) in small neuronal compartments is crucial to understanding Ca2+-dependent signaling. Here, we describe techniques that are optimized for 2-photon imaging of [Ca2+] dynamics in small compartments such as dendrites and dendritic spines.

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AnimalsCalcium SignalingCell CompartmentationDendritesHippocampusImage Processing, Computer-AssistedLasersMicroscopy, Fluorescence, MultiphotonNeuronsPatch-Clamp TechniquesRatsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Ryohei Yasuda, Esther A Nimchinsky, Volker Scheuss, Thomas A Pologruto, Thomas G Oertner, Bernardo L Sabatini, Karel Svoboda. 2004-02-03. Imaging calcium concentration dynamics in small neuronal compartments.. https://doi.org/10.1126/stke.2192004pl5

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