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PubMed · 1489098

Characterization of immune complex components by dot blot analysis.

W L Hoffman·P J Kelly·A O Ruggles
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Abstract

A method is described for the characterization of immune complex components by dot blot analysis. After isolation by chromatographic techniques and precipitation with polyethylene glycol, immune complexes were dissociated in 0.1 M phosphate (pH 2) and bound to a nitrocellulose membrane in a dot blot unit. Biotinylated probes were then used to identify the following immune complex components: specific antigens, biologically active antibodies, antibody isotypes, antibody subclasses, antibody idiotypes, and rheumatoid factors. This nonradioactive procedure takes less than 2 h to perform and has been used to analyze immune complexes isolated from sera (rabbit and human) and synovial fluid (human).

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AcidsAnimalsAntibodies, Anti-IdiotypicAntibodies, MonoclonalAntigen-Antibody ComplexArthritis, RheumatoidBuffersChemical PrecipitationCollodionComplement C1qElectrophoresisHumansHydrogen-Ion ConcentrationImmunoblottingImmunoglobulin GImmunoglobulin IsotypesOvalbuminRabbitsSynovial FluidComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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W L Hoffman, P J Kelly, A O Ruggles. 1992-11-15. Characterization of immune complex components by dot blot analysis.. https://doi.org/10.1016/0003-2697(92)90497-u

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