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Improved flow cytometric method for HLA-B27 typing.

W C Janssen·J A Rouwen·J J Hoffmann
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Abstract

HLA-B27 is a cell marker of clinical interest because of its high association with certain diseases. The HLA-B27 antigen was detected on lymphocytes using a monoclonal antibody in an indirect immuno-fluorescence assay using a fluorescence flow cytometer. The considerable crossreaction of the monoclonal antibody with the HLA-B7 antigen was effectively suppressed by masking it by means of human anti-HLA-B7 antiserum. The flow cytometric method was evaluated by comparing the results with those obtained by the standard lymphocytotoxicity test and showed complete agreement in 107 selected patient samples.

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Cross ReactionsFlow CytometryFluorescent Antibody TechniqueHLA-B27 AntigenHLA-B7 AntigenHumansPhenotypeJournal Article

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W C Janssen, J A Rouwen, J J Hoffmann. 1992. Improved flow cytometric method for HLA-B27 typing.. https://doi.org/10.1177/000456329202900608

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