Absolute expression values for mouse transcripts: re-annotation of the READ expression database by the use of CAGE and EST sequence tags.

Rimantas Kodzius; Yonehiro Matsumura; Takeya Kasukawa; Kazuro Shimokawa; Shiro Fukuda; Toshiyuki Shiraki; Mari Nakamura; Takahiro Arakawa; Daisuke Sasaki; Jun Kawai; Matthias Harbers; Piero Carninci; Yoshihide Hayashizaki

doi:10.1016/s0014-5793(04)00018-3

PubMed · 14960301

Absolute expression values for mouse transcripts: re-annotation of the READ expression database by the use of CAGE and EST sequence tags.

Rimantas Kodzius·Yonehiro Matsumura·Takeya Kasukawa·Kazuro Shimokawa·Shiro Fukuda·Toshiyuki Shiraki·Mari Nakamura·Takahiro Arakawa·Daisuke Sasaki·Jun Kawai·Matthias Harbers·Piero Carninci·Yoshihide Hayashizaki

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Abstract

The RIKEN expression array database (READ) provides comprehensive gene expression data for the mouse, which were obtained as relative values from microarray double-staining experiments with E17.5 mRNA as common reference. To assign absolute expression values for mouse transcripts within READ, we applied the E17.5 reference sample to CAGE (cap analysis of gene expression) and expressed sequence tag (EST) high-throughput tag sequencing. Newly assigned values within the READ database were validated by comparison to expression data from serial analysis of gene expression, CAGE and EST experiments. These experiments confirmed the great significance of the absolute expression values within the improved READ database. The new Absolute READ database on absolute expression data is available under.

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Animals Databases, Nucleic Acid Expressed Sequence Tags Gene Expression Profiling Mice Mice, Inbred C57BL Oligonucleotide Array Sequence Analysis Organ Specificity RNA Caps RNA, Messenger Journal Article Research Support, Non-U.S. Gov't

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Rimantas Kodzius, Yonehiro Matsumura, Takeya Kasukawa, Kazuro Shimokawa, Shiro Fukuda, Toshiyuki Shiraki, Mari Nakamura, Takahiro Arakawa, Daisuke Sasaki, Jun Kawai, Matthias Harbers, Piero Carninci, Yoshihide Hayashizaki. 2004-02-13. Absolute expression values for mouse transcripts: re-annotation of the READ expression database by the use of CAGE and EST sequence tags.. https://doi.org/10.1016/s0014-5793(04)00018-3

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