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Optimal enzymes for single-molecule sequencing.

Susanne Brakmann
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Abstract

Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemist's point of view.

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AnimalsDNA-Directed DNA PolymeraseFluorescent DyesHumansSequence Analysis, DNAJournal ArticleReview

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Susanne Brakmann. 2004. Optimal enzymes for single-molecule sequencing.. https://doi.org/10.2174/1389201043489611

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