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PubMed · 14978528

Genotyping using single nucleotide polymorphism, fluorescence spectroscopy and pattern recognition.

Richard G Brereton·Martin Devonshire
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Abstract

This paper describes a method for genetic screening using single nucleotide polymorphism. Fluorescence spectra with an excitation frequency of 488 nm are recorded over a range of 550 to 660 nm of fragments of human DNA together with two fluorescent probe dyes attached to specific primers, one for each type of allele and a background dye, prepared using the Taqman reaction. The fluorescence spectra are monitored and principal components analysis used to separate spectra into three groups, which are visually identified as allele 1 (wild type), allele 2 (mutant) and mixed allele by comparison to reference samples. Malahanobis distance using 4 principal components are used to correctly classify samples into groups.

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DNAGenetic TestingGenotypeHumansMicroscopy, FluorescencePattern Recognition, AutomatedPolymorphism, Single NucleotideJournal Article

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Richard G Brereton, Martin Devonshire. 2004-01-22. Genotyping using single nucleotide polymorphism, fluorescence spectroscopy and pattern recognition.. https://doi.org/10.1039/b307176f

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