A simple and efficient method for isolating trichomes for downstream analyses.

Xiaoguo Zhang; David G Oppenheimer

doi:10.1093/pcp/pch016

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A simple and efficient method for isolating trichomes for downstream analyses.

Xiaoguo Zhang·David G Oppenheimer

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Abstract

Arabidopsis trichomes are an excellent cell type to address many questions in plant biology including the control of cell shape, endoreplication, and cell expansion. Because trichomes comprise such a small percentage of the cells of a leaf, biochemical analyses of trichomes are limited. To overcome this limitation, we developed a method for removing trichomes from the leaf surface. Our method allows the isolation of intact trichomes for use in downstream applications such as cell wall analysis, immunolocalization of trichome proteins, analysis of DNA content, and proteomics. Also, this method will facilitate the isolation of trichomes from practically any plant species.

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Arabidopsis Calcium Cell Adhesion Cell Separation Cell Wall Centrifugation Chelating Agents Cytological Techniques DNA, Plant Egtazic Acid Microscopy, Electron, Scanning Octoxynol Plant Leaves Plant Proteins Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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Xiaoguo Zhang, David G Oppenheimer. 2004. A simple and efficient method for isolating trichomes for downstream analyses.. https://doi.org/10.1093/pcp%2Fpch016

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