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PubMed · 14989097

Comparison of different probe-level analysis techniques for oligonucleotide microarrays.

Barbara Rosati·Frederic Grau·Anneke Kuehler·Samantha Rodriguez·David McKinnon
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Abstract

Three different software packages for the probe-level analysis of high-density oligonucleotide microarray data were compared using an experiment-derived data set that was validated using real-time PCR. The efficiency with which these three programs could identify true positives in this data set was assessed. In addition, estimates of false-positive and false-negative rates were determined. The performance of the programs using very small data sets was also compared, and recommendations for use are suggested.

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AnimalsFalse Negative ReactionsFalse Positive ReactionsGene Expression ProfilingMyocardiumOligonucleotide Array Sequence AnalysisOligonucleotide ProbesPolymerase Chain ReactionRatsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Barbara Rosati, Frederic Grau, Anneke Kuehler, Samantha Rodriguez, David McKinnon. 2004. Comparison of different probe-level analysis techniques for oligonucleotide microarrays.. https://doi.org/10.2144/04362mt03

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