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Improved quantification limits in chiral capillary electrochromatography by peak compression effects.

Anna Maria Enlund·Margareta E Andersson·Gunnar Hagman
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Abstract

The peak compression effect has been applied to improve quantification limits in chiral capillary electrochromatography (CEC). A stationary phase based on the chiral selector vancomycin (Chirobiotic V) was used for separations of the enantiomers of mianserin. By adding solvents with a low dielectric constant, e.g. 2-propanol or tetrahydrofuran, to the sample solution, peak compression could be induced. The plate numbers for the minor enantiomer increased from approximately 100,000 to 1.4-1.6 million plates/m, when the composition of the mobile phase was adjusted so that the analyte eluted within either one of two system zones originating from the sample solution. A 10-fold improvement in the quantification limit for the minor enantiomer was obtained compared to elution under non-focused conditions.

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2-PropanolBuffersElectrophoresis, CapillaryFuransHydrogen-Ion ConcentrationMianserinSolventsStereoisomerismVancomycinJournal Article

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Anna Maria Enlund, Margareta E Andersson, Gunnar Hagman. 2004-03-05. Improved quantification limits in chiral capillary electrochromatography by peak compression effects.. https://doi.org/10.1016/j.chroma.2003.11.107

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