FlyGEM, a full transcriptome array platform for the Drosophila community.

Rick Johnston; Bruce Wang; Rachel Nuttall; Michael Doctolero; Pamela Edwards; Jining Lü; Marina Vainer; Huibin Yue; Xinhao Wang; James Minor; Cathy Chan; Alex Lash; Thomas Goralski; Michael Parisi; Brian Oliver; Scott Eastman

doi:10.1186/gb-2004-5-3-r19

PubMed · 15003122

FlyGEM, a full transcriptome array platform for the Drosophila community.

Rick Johnston·Bruce Wang·Rachel Nuttall·Michael Doctolero·Pamela Edwards·Jining Lü·Marina Vainer·Huibin Yue·Xinhao Wang·James Minor·Cathy Chan·Alex Lash·Thomas Goralski·Michael Parisi·Brian Oliver·Scott Eastman

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Abstract

We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.

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Animals Blotting, Northern DNA DNA Primers DNA Probes DNA, Complementary Drosophila melanogaster Exons Fluorescent Dyes Gene Expression Profiling Genes, Insect Genome Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis RNA Reproducibility of Results Comparative Study Journal Article

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BibTeX RIS

Rick Johnston, Bruce Wang, Rachel Nuttall, Michael Doctolero, Pamela Edwards, Jining Lü, Marina Vainer, Huibin Yue, Xinhao Wang, James Minor, Cathy Chan, Alex Lash, Thomas Goralski, Michael Parisi, Brian Oliver, Scott Eastman. 2004-02-26. FlyGEM, a full transcriptome array platform for the Drosophila community.. https://doi.org/10.1186/gb-2004-5-3-r19

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