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PubMed · 15003207

Sequencing the maize genome.

Robert A Martienssen·Pablo D Rabinowicz·Andrew O'Shaughnessy·W Richard McCombie
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Abstract

Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads. Simulations using sequenced bacterial artificial chromosome (BAC) clones predict that 5x coverage of gene-rich regions, accompanied by less than 1x coverage of subclones from BAC contigs, will generate high-quality mapped sequence that meets the needs of geneticists while accommodating unusually high levels of structural polymorphism. By sequencing several inbred strains, we propose a strategy for capturing this polymorphism to investigate hybrid vigor or heterosis.

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Chromosomes, Artificial, BacterialGenome, PlantPhysical Chromosome MappingZea maysJournal ArticleResearch Support, Non-U.S. Gov'tReview

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Robert A Martienssen, Pablo D Rabinowicz, Andrew O'Shaughnessy, W Richard McCombie. 2004. Sequencing the maize genome.. https://doi.org/10.1016/j.pbi.2004.01.010

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