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PubMed · 15003551

Gene expression in the developing rat mandible: a gene array study.

Maiko Oshikawa·Naoyuki Sugano·Ryo Ishigaki·Koichi Ito
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Abstract

To analyse the molecular events that occur in the developing mandible, gene arrays containing probes for 1176 known genes were used. Total RNA was extracted from the mandibles of 1- and 6-day-old rats. Radiolabeled probes were then synthesised and used to probe the DNA arrays. Of the 1176 genes examined, 306 were detectable, and the latter were analyzed by bioinformatics algorithms. K-means clustering grouped 72 genes into Up, 56 genes into Down and 178 genes into NO change. A large number of genes related to cell receptors (by ligands) were grouped into the Down cluster. Gene array technology appears to be a useful tool for studying the complex process of mandibular development.

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AnimalsGene ExpressionMandibleOligonucleotide Array Sequence AnalysisRNARatsRats, WistarJournal ArticleResearch Support, Non-U.S. Gov't

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Maiko Oshikawa, Naoyuki Sugano, Ryo Ishigaki, Koichi Ito. 2004. Gene expression in the developing rat mandible: a gene array study.. https://doi.org/10.1016/j.archoralbio.2003.09.008

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