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PubMed · 15004843

High-throughput single nucleotide polymorphism typing by fluorescent single-strand conformation polymorphism analysis with capillary electrophoresis.

Kent Doi·Hitomi Doi·Eisei Noiri·Akihide Nakao·Toshiro Fujita·Katsushi Tokunaga
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Abstract

In this study, we performed high-throughput and precise single nucleotide polymorphism (SNP) typing by fluorescent capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) analysis. A system composed of a multicapillary DNA analyzer, a newly developed sieving matrix, four different colors of fluorescent labels, and a multiplex polymerase chain reaction (PCR) enabled low-cost and highly reliable SNP typing. Moreover, this system enabled the estimation of SNP allele frequencies using pooled DNA samples, which should be beneficial for large-scale association studies. Thus, fluorescent CE-SSCP analysis is a useful method for large-scale SNP typing.

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DNA FingerprintingElectrophoresis, CapillaryFluorescent DyesGene FrequencyHumansPolymerase Chain ReactionPolymorphism, Single NucleotidePolymorphism, Single-Stranded ConformationalSequence Analysis, DNAStaining and LabelingEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Kent Doi, Hitomi Doi, Eisei Noiri, Akihide Nakao, Toshiro Fujita, Katsushi Tokunaga. 2004. High-throughput single nucleotide polymorphism typing by fluorescent single-strand conformation polymorphism analysis with capillary electrophoresis.. https://doi.org/10.1002/elps.200305721

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