Molecular-based methods for quantifying HIV viral load.

James B Peter; J Sanders Sevall

doi:10.1089/108729104322802506

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PubMed · 15006182

Molecular-based methods for quantifying HIV viral load.

James B Peter·J Sanders Sevall

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Abstract

Viral load quantitation has become the major prognostic marker for disease prognosis and outcome of antiretroviral therapy in the treatment of HIV-infected individuals. The three major methodologies for viral load quantitation: the reverse transcriptase-polymerase chain reaction (RT-PCR; Amplicor HIV-1 Monitor Test, Roche Diagnostic Systems, Pleasanton, CA), the nucleic acid sequence-based amplification (NASBA; NucliSens HIV-1 QT Test, Organon Teknika, Bostel, The Netherlands); and a signal amplification methodology termed branchedchain DNA (bDNA) technique (Quantiplex HIV-1 RNA test, Bayer Diagnostics, Emeryville, CA) are briefly reviewed here.

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Biomarkers Branched DNA Signal Amplification Assay HIV Infections HIV-1 Humans Nucleic Acid Amplification Techniques Prognosis RNA, Viral Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Viral Load Comparative Study Journal Article Review

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James B Peter, J Sanders Sevall. 2004. Molecular-based methods for quantifying HIV viral load.. https://doi.org/10.1089/108729104322802506

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