Streptococcus mutans fructosyltransferase interactions with glucans.

Ramona Rozen; Doron Steinberg; Gilad Bachrach

doi:10.1016/s0378-1097(04)00065-5

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PubMed · 15019732

Streptococcus mutans fructosyltransferase interactions with glucans.

Ramona Rozen·Doron Steinberg·Gilad Bachrach

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Abstract

Streptococcus mutans utilizes sucrose to synthesize glucans by glucosyltransferase and fructans by fructosyltransferase (FTF). Antibodies raised against a recombinant FTF were used to study S. mutans FTF secretion. Low amounts of cell-free FTF were found in culture of S. mutans grown with sucrose, while an increase in bacteria displaying cell surface FTF was detected. FTF added to S. mutans cultures was adsorbed to bacteria grown with sucrose but not to bacteria grown with glucose or fructose or to a gtf inactivated mutant grown with sucrose. Recombinant FTF was found to have high affinity for glucans suggesting that fructans and glucans are an integral part of the polysaccharide matrix of oral biofilms.

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Biofilms Culture Media Fructans Fructose Gene Deletion Glucans Glucose Glucosyltransferases Hexosyltransferases Polysaccharides, Bacterial Protein Binding Recombinant Proteins Streptococcus mutans Sucrose Bacterial Proteins Journal Article

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Ramona Rozen, Doron Steinberg, Gilad Bachrach. 2004-03-12. Streptococcus mutans fructosyltransferase interactions with glucans.. https://doi.org/10.1016/s0378-1097(04)00065-5

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