Protein arrays from cDNA expression libraries.

Hendrik Weiner; Thomas Faupel; Konrad Büssow

doi:10.1385/1-59259-759-9:001

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PubMed · 15020775

Protein arrays from cDNA expression libraries.

Hendrik Weiner·Thomas Faupel·Konrad Büssow

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Abstract

This chapter describes the production of a cDNA expression library from human fetal brain, the construction of a high-density protein array from such a library, and two applications to screen the array for binding proteins. After producing the library and decollating the expression clones, one can pick thousands of expression clones with a laboratory robot and can deposit them into microtiter plates in an ordered manner. Such ordered clone libraries are the starting material for the construction of a high-density protein array. This array is constructed by spotting the expression clones onto a protein-binding membrane. Following cell growth and induction of protein expression on the membrane, the cell spots are lysed and their recombinant protein immobilized on the membrane. The so-constructed array carries thousands of proteins without the need to clone, express, and spot individual proteins. Such arrays allow one to screen for numerous protein functions in a high-throughput manner.

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Amino Acid Sequence Base Sequence Brain Chemistry Cloning, Molecular Fetus Gene Library Humans Molecular Sequence Data Protein Array Analysis Protein Binding Recombinant Proteins Journal Article

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Hendrik Weiner, Thomas Faupel, Konrad Büssow. 2004. Protein arrays from cDNA expression libraries.. https://doi.org/10.1385/1-59259-759-9%3A001

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