A streptavidin-biotin-based microarray platform for immunoassays.

Petra Pavlickova; Hubert Hug

doi:10.1385/1-59259-759-9:073

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A streptavidin-biotin-based microarray platform for immunoassays.

Petra Pavlickova·Hubert Hug

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Abstract

The authors describe a microarray system for disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.

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Animals Antigens Biotin Gold Humans Immunoassay Immunoglobulin Fragments Immunoglobulin M Lyme Disease Peptides Protein Array Analysis Reproducibility of Results Streptavidin Journal Article Research Support, Non-U.S. Gov't

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Petra Pavlickova, Hubert Hug. 2004. A streptavidin-biotin-based microarray platform for immunoassays.. https://doi.org/10.1385/1-59259-759-9%3A073

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