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PubMed · 15020788

A protein microarray ELISA for screening biological fluids.

Susan M Varnum·Ronald L Woodbury·Richard C Zangar
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Abstract

Protein microarrays permit the simultaneous measurement of many proteins in a small sample volume and therefore provide an attractive approach for the quantitative measurement of proteins in biological fluids, including serum. This chapter describes a microarray enzyme-linked immunosorbent assay (ELISA). Capture antibodies are immobilized onto a glass surface; the covalently attached antibodies bind a specific antigen from a sample overlaying the array. A second, biotinylated antibody that recognizes the same antigen as the first antibody, but at a different epitope, is then used for detection. Detection is based on an enzymatic signal-enhancement method known as tyramide signal amplification (TSA). By coupling a microarray-ELISA format with the signal amplification of tyramide deposition, the assay sensitivity is as low as sub-pg/mL.

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AmidesAntibodiesAntibody AffinityBiotinBody FluidsEnzyme-Linked Immunosorbent AssayEpitopesProtein Array AnalysisProteinsReproducibility of ResultsSensitivity and SpecificityStreptavidinJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Susan M Varnum, Ronald L Woodbury, Richard C Zangar. 2004. A protein microarray ELISA for screening biological fluids.. https://doi.org/10.1385/1-59259-759-9%3A161

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