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Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin.

M G Mage·L L McHugh·T L Rothstein
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Abstract

Mouse spleen cells could be preparatively separated into immunoglobulin positive (Ig+) and immunoglobulin-netative (Ig-)populations by incubating as many as 2 X 10(8) cells per 100 mm diameter petri plate coated with specifically purified goat anti-mouse immunoglobulin. The non-adherent population was 95% or more Ig-, and possessed graft versus host and cytotoxic effector activities, as would be expected for T cells. They could also give a mixed lymphocyte reaction and generate cytotoxic effector activity on culture in vitro. The adherent cells could not be released undamaged from plates coated with undiluted anti-Ig, but they could be released from plates coated with a 1/4 or 1/10 dilution of anti-Ig in an irrelevant antibody. The released cells were over 90% viable by trypan-blue staining, and 94% or more of the viable cells were Ig+.

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AnimalsAntibodies, Anti-IdiotypicAntibody SpecificityBinding Sites, AntibodyCell AdhesionCell SeparationCytotoxicity Tests, ImmunologicGoatsGraft vs Host ReactionImmune SeraImmunity, CellularLymphocyte Culture Test, MixedLymphocytesMiceMice, Inbred BALB CMice, Inbred C57BLPolystyrenesReceptors, Antigen, B-CellSpleenThymus GlandJournal Article

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M G Mage, L L McHugh, T L Rothstein. 1977. Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin.. https://doi.org/10.1016/0022-1759(77)90016-3

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