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Two-dimensional diversity: screening human cDNA phage display libraries with a random diversity probe for the display cloning of phosphotyrosine binding domains.

Elizabeth J Videlock·Victor K Chung·Michael A Mohan·Timothy M Strok·David J Austin
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Abstract

A random phosphopeptide probe (bio-pYZZZ) has been used for the isolation and identification of multiple SH2 domains from human cDNA-displaying phage libraries. In addition, on-phage analysis and quantification of binding affinities for these phage-displayed proteins has shown them to be functional domains, retaining the same characteristics as in their native state.

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Cloning, MolecularColiphagesGene LibraryHumansLigandsMolecular StructurePeptide FragmentsPhosphorylationPhosphotyrosineProtein BindingSignal Transductionsrc Homology DomainsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Elizabeth J Videlock, Victor K Chung, Michael A Mohan, Timothy M Strok, David J Austin. 2004-03-31. Two-dimensional diversity: screening human cDNA phage display libraries with a random diversity probe for the display cloning of phosphotyrosine binding domains.. https://doi.org/10.1021/ja039006p

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