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Linear phase imaging using differential interference contrast microscopy.

M R Arnison·K G Larkin·C J R Sheppard·N I Smith·C J Cogswell
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Abstract

We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.

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Fourier AnalysisImage Processing, Computer-AssistedMicroscopy, Phase-ContrastModels, TheoreticalJournal ArticleResearch Support, Non-U.S. Gov't

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M R Arnison, K G Larkin, C J R Sheppard, N I Smith, C J Cogswell. 2004. Linear phase imaging using differential interference contrast microscopy.. https://doi.org/10.1111/j.0022-2720.2004.01293.x

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