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A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.

M C Serrano·M Ramírez·D Morilla·A Valverde·M Chávez·A Espinel-Ingroff·R Claro·A Fernández·C Almeida·E Martín-Mazuelos
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Abstract

OBJECTIVE: The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods. METHODS: Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller-Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges. RESULTS: The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods. CONCLUSION: On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.

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Antifungal AgentsAspergillusCulture MediaDiffusionMicrobial Sensitivity TestsPyrimidinesQuality ControlTriazolesVoriconazoleComparative StudyJournal Article

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M C Serrano, M Ramírez, D Morilla, A Valverde, M Chávez, A Espinel-Ingroff, R Claro, A Fernández, C Almeida, E Martín-Mazuelos. 2004-03-31. A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.. https://doi.org/10.1093/jac%2Fdkh172

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