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Yeast differentiation using histone promoter sequences.

P J L Bell
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Abstract

AIMS: To evaluate a new platform for yeast differentiation based on histone promoter regions. METHODS AND RESULTS: The histone gene amino acid sequences of a wide phylogenetic range of organisms were aligned, and primers designed that were capable of amplifying the divergent promoters of the H3-H4 and H2a-H2b loci from yeast. Analysis indicated that the promoter regions were variable in length between species and represented rapidly changing sequences flanked by highly conserved sequences. CONCLUSIONS: The histone promoter regions in yeast provide an excellent locus for the rapid and accurate identification of yeast species. SIGNIFICANCE AND IMPACT OF THE STUDY: This study describes an alternative platform to the ribosomal internal transcribed spacer (ITS) sequences for the identification of yeast species.

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Base SequenceDNA, FungalHistonesMolecular Sequence DataMycological Typing TechniquesPolymerase Chain ReactionPromoter Regions, GeneticSaccharomycetalesEvaluation StudyJournal Article

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P J L Bell. 2004. Yeast differentiation using histone promoter sequences.. https://doi.org/10.1111/j.1472-765x.2004.01508.x

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