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PubMed · 15067873

Microarray-based in vitro evaluation of DNA oligomer libraries designed in silico.

Udo Feldkamp·Ron Wacker·Hendrik Schroeder·Wolfgang Banzhaf·Christof M Niemeyer
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Abstract

We report on the microarray-based in vitro evaluation of two libraries of DNA oligonucleotide sequences, designed in silico for applications in supramolecular self-assembly, such as DNA computing and DNA-based nanosciences. In this first study which is devoted to the comparison of sequence motif properties theoretically predicted with their performance in real-life, the DNA-directed immobilization (DDI) of proteins was used as an example of DNA-based self-assembly. Since DDI technologies, DNA computing, and DNA nanoconstruction essentially depend on similar prereguisites, in particular, large and uniform hybridization efficiencies combined with low nonspecific cross-reactivity between individual sequences, we anticipate that the microarray approach demonstrated here will enable rapid evaluation of other DNA sequence libraries.

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Base SequenceComputational BiologyDatabases as TopicGene LibraryImage Processing, Computer-AssistedMicroscopy, FluorescenceModels, BiologicalNanotechnologyNucleic Acid HybridizationOligodeoxyribonucleotidesOligonucleotide Array Sequence AnalysisComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Udo Feldkamp, Ron Wacker, Hendrik Schroeder, Wolfgang Banzhaf, Christof M Niemeyer. 2004-03-19. Microarray-based in vitro evaluation of DNA oligomer libraries designed in silico.. https://doi.org/10.1002/cphc.200300978

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