High throughput screening technologies for direct cyclic AMP measurement.

Daniela Gabriel; Magali Vernier; Martin J Pfeifer; Boris Dasen; Laurent Tenaillon; Rochdi Bouhelal

doi:10.1089/15406580360545107

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High throughput screening technologies for direct cyclic AMP measurement.

Daniela Gabriel·Magali Vernier·Martin J Pfeifer·Boris Dasen·Laurent Tenaillon·Rochdi Bouhelal

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Abstract

A study comparing five different cAMP detection technologies in terms of sensitivity, robustness, and feasibility for HTS is presented. In this report, the following methods are described: a nonhomogeneous DELFIA, and the homogeneous methods based on time-resolved fluorescence (HTRF), luminescent singlet oxygen channeling or ALPHAScreen, FP, and high-affinity enzyme complementation. DELFIA had the highest sensitivity, whereas ALPHAScreen and HTRF shared several advantages, including high sensitivity, broad dynamic range, and minimal reagent addition steps. For G(s)-coupled antagonist screens, we found HTRF and ALPHAScreen the more sensitive and HTS-compatible techniques.

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Animals Biological Assay CHO Cells Colforsin Cricetinae Cyclic AMP Fluorescence Fluoroimmunoassay GTP-Binding Proteins Gene Expression Luminescent Measurements Reagent Kits, Diagnostic Technology, Pharmaceutical Comparative Study Journal Article

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Daniela Gabriel, Magali Vernier, Martin J Pfeifer, Boris Dasen, Laurent Tenaillon, Rochdi Bouhelal. 2003. High throughput screening technologies for direct cyclic AMP measurement.. https://doi.org/10.1089/15406580360545107

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