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Regional differences in blood-nerve barrier function and tight-junction protein expression within the rat dorsal root ganglion.

Hisanori Hirakawa·Seiichiro Okajima·Takanori Nagaoka·Toshikazu Kubo·Tetsuro Takamatsu·Masahito Oyamada
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Abstract

To elucidate blood-nerve barrier function and tight-junction protein expression in the dorsal root ganglion (DRG), we analyzed the vascular permeability in the rat DRG by i.v. administration of fluorescent Evans-blue albumin (EBA) and compared it with the localization of claudin-1, claudin-5, and occludin by immunoconfocal microscopy. In the cell body-rich area within the DRG, extravascular leakage of EBA was noted and claudin-5 but neither claudin-1 nor occludin was detected. Conversely, in the nerve fiber-rich area within the DRG, no extravascular leakage of EBA was observed and both claudin-5 and occludin but no claudin-1 were detected in the blood vessel. These results demonstrate regional differences in the blood-nerve barrier function and tight-junction protein expression within the DRG.

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AnimalsCapillary PermeabilityClaudin-1Claudin-5Evans BlueFluorescent DyesGanglia, SpinalImmunohistochemistryMaleMembrane ProteinsMicroscopy, ConfocalNerve Tissue ProteinsOccludinRatsRats, WistarTight Junctionsvon Willebrand FactorJournal ArticleResearch Support, Non-U.S. Gov't

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Hisanori Hirakawa, Seiichiro Okajima, Takanori Nagaoka, Toshikazu Kubo, Tetsuro Takamatsu, Masahito Oyamada. 2004-03-01. Regional differences in blood-nerve barrier function and tight-junction protein expression within the rat dorsal root ganglion.. https://doi.org/10.1097/00001756-200403010-00004

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