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PubMed · 15099517

Gene-specific modulation of TAF10 function by SET9-mediated methylation.

Antigone Kouskouti·Elisabeth Scheer·Adrien Staub·Làszlò Tora·Iannis Talianidis
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Abstract

SET9 is a member of the SET domain-containing histone methyltransferase family that can specifically methylate histone 3 at lysine 4 position. Although nucleosomal histones are poor substrates for SET9, the active enzyme can stimulate activator-induced transcription. Here, we show that SET9 can monomethylate the TBP-associated factor TAF10 at a single lysine residue located at the loop 2 region within the putative histone-fold domain of the protein. Methylated TAF10 has an increased affinity for RNA polymerase II, pointing to a direct role of this modification in preinitiation complex formation. Reporter assays and studies on TAF10 null F9 cells expressing a methylation-deficient TAF10 mutant revealed that SET9-mediated methylation of TAF10 potentiates transcription of some but not all TAF10-dependent genes. This gene specificity correlated with SET9 recruitment. The promoter-specific effects of SET9-methylated TAF10 may have important implications regarding the biological function of SET domain-containing lysine methylases, whose primary targets have been presumed to be histones.

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Amino Acid SequenceAntibodies, MonoclonalBaculoviridaeBinding SitesCell LineCell NucleusEscherichia coliGene Expression RegulationGenes, ReporterGenetic VectorsHeLa CellsHistonesHumansLysineMethylationMethyltransferasesMutationNucleosomesPrecipitin TestsProtein Structure, TertiaryProteinsRNA Polymerase IIRecombinant Fusion ProteinsSubstrate SpecificityTATA-Binding Protein Associated FactorsTime FactorsTranscription Factor TFIIDTranscription, GeneticTranscriptional ActivationJournal ArticleResearch Support, Non-U.S. Gov't

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Antigone Kouskouti, Elisabeth Scheer, Adrien Staub, Làszlò Tora, Iannis Talianidis. 2004-04-23. Gene-specific modulation of TAF10 function by SET9-mediated methylation.. https://doi.org/10.1016/s1097-2765(04)00182-0

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