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Synaptobrevin transmembrane domain dimerization-revisited.

Rana Roy·Rico Laage·Dieter Langosch
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Abstract

Synaptobrevin is a membrane-spanning soluble N-ethyl maleimid-sensitive factor (NSF) attachment protein receptor (SNARE) protein of synaptic vesicles that is essential for neurotransmitter release. Various lines of evidence indicate that it exists alternatively as a monomer, as a homodimer, as a heterodimer with synaptophysin, or as a ternary complex with other SNAREs at the various stages of the synaptic vesicle cycle. Homodimerization of synaptobrevin was previously shown by different authors to depend on its single transmembrane segment, and the crucial residues forming the helix-helix interface have been mapped. Since another recent study challenged these results, we reinvestigated this issue. Here, we show that native synaptobrevin can be cross-linked in synaptic vesicle membranes to a homodimer by disulfide bond formation between cysteine residues of the transmembrane segment. Further, we demonstrate that determination of synaptobrevin transmembrane segment interactions in membranes or in detergent solution requires careful control of experimental conditions. Thus, our present results corroborate that homodimerization of synaptobrevin is mediated by its transmembrane segment.

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Amino Acid SequenceAnimalsBlotting, WesternCircular DichroismConserved SequenceCross-Linking ReagentsCysteineDimerizationDisulfidesElectrophoresis, Polyacrylamide GelMaleMembrane ProteinsModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedProtein Structure, SecondaryProtein Structure, TertiaryR-SNARE ProteinsRatsRats, WistarRecombinant Fusion ProteinsSNARE ProteinsSequence Homology, Amino AcidSynaptic MembranesVesicular Transport ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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Rana Roy, Rico Laage, Dieter Langosch. 2004-05-04. Synaptobrevin transmembrane domain dimerization-revisited.. https://doi.org/10.1021/bi0362875

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