PubMed · 15116338
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Athanase Billis. 2004. Intraprostatic fat: does it exist?. https://doi.org/10.1016/j.humpath.2003.12.003
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The accessibility of adipose tissue and its ability to secrete various bioactive molecules suggest that adipose cells may be attractive targets for gene therapy applications. Here, we report the use of highly defective herpes simplex virus (HSV) vectors as suitable gene transfer agents for adipose cells in culture and fat tissue in animals. Using an in vitro model of human adipose differentiation, we first demonstrated that mature adipocytes and their precursor cells express the two principal HSV viral entry receptors HveA and HveC (nectin-1) and are efficiently transduced at a low multiplicity of infection by HSV-lacZ reporter gene and glial cell line-derived neurotrophic factor (GDNF) gene vectors. Extended expression of beta-galactosidase and secretion of GDNF occurred in transduced fat tissue explants from rabbits. In vivo gene transfer to rabbit subcutaneous adipose tissue resulted in local GDNF expression for at least 2 months. These experiments establish the efficient transduction of adipose cells by HSV vectors and suggest that fat tissue may represent a useful site for HSV-mediated gene delivery with potential for therapeutic applications.
Normal human and mouse bone marrow is composed of hematopoietic and nonhematopoietic cells. The latter have also been termed stromal cells, microenvironment cells, colony-forming-unit fibroblasts (CFU-F), and mesenchymal cells. These cells were originally thought to provide an appropriate matrix for hematopoietic cell development, but recent examination of these cell populations suggests a much broader spectrum of activity, including the generation of bone, cartilage, muscle, tendon, and fat. In the future, these mesenchymal cell populations could be used for the treatment of specific diseases and to enhance the engraftment of hematopoietic cells. This chapter describes methods for the human CFU-F assay, culture and expansion of mesenchymal cells, as well as their differentiation to adipocytes. In addition, this chapter describes the mouse CFU-F assay.
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