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PubMed · 1511991

A polymorphic complex dinucleotide repeat at the telomeric D8S7 locus.

S Wood·M Schertzer
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Abstract

Two cosmids isolated from a flow-sorted chromosome 8 library by hybridization with pSW50 (D8S7) were screened for GT microsatellite sequences. Both contained a positive 900-bp Hind III-Xba I fragment. Sequencing revealed a complex dinucleotide repeat. Flanking oligonucleotide primers were synthesized, and the polymerase chain reaction products produced by these primers were typed within the CEPH panel of families. A two-allele polymorphism was identified that is in linkage disequilibrium with a previously described insertion-deletion polymorphism at this locus.

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Base SequenceChromosome MappingChromosomes, Human, Pair 8Deoxyribonuclease HindIIIGenetic LinkageHumansMolecular Sequence DataOligonucleotidesPedigreePolymerase Chain ReactionPolymorphism, GeneticRepetitive Sequences, Nucleic AcidTelomereJournal ArticleResearch Support, Non-U.S. Gov't

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S Wood, M Schertzer. 1992. A polymorphic complex dinucleotide repeat at the telomeric D8S7 locus.. https://doi.org/10.1159/000154057

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