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PubMed · 15130844

MProbe: computer aided probe design for oligonucleotide microarrays.

Wuju Li·Jian Huang·Ming Fan·Shenqi Wang
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Abstract

The present work describes a complete probe design software system for oligonucleotide microarrays based on Kane's research on probe sensitivity and specificity (Kane's rule). Combining Kane's rule and traditional criteria for probe design we constructed MProbe, the software system for oligonucleotide microarrays using Java. The general criteria for probe design are: (1) probes may have different lengths that range from 20 to 100 bases; (2) they should have a similar melting temperature (Tm) or GC content; (3) they should not contain stable secondary structures; and (4) they abide by Kane's rule.

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Computational BiologyComputer-Aided DesignDNA ProbesDrug DesignOligonucleotide Array Sequence AnalysisSoftwareJournal ArticleResearch Support, Non-U.S. Gov't

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Wuju Li, Jian Huang, Ming Fan, Shenqi Wang. 2002. MProbe: computer aided probe design for oligonucleotide microarrays.. https://pubmed.ncbi.nlm.nih.gov/15130844/

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