Affinity adsorbent based on combinatorial phage display peptides that bind alpha-cobratoxin.

Woo-Hyeon Byeon; Bernard Weisblum

doi:10.1016/j.jchromb.2004.03.019

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Affinity adsorbent based on combinatorial phage display peptides that bind alpha-cobratoxin.

Woo-Hyeon Byeon·Bernard Weisblum

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Abstract

Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to agarose through the alpha-amino terminus. Such affinity chromatography supports selectively bound the alpha-cobratoxin component from crude venom, while passage of the crude venom over the support selectively depleted the venom of this component. The selective binding of alpha-cobratoxin to peptide-based solid-phase supports suggests that a limitless variety of peptides similarly obtained by combinatorial phage display can be used to craft specific analytical and preparative tools.

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Adsorption Amino Acid Sequence Bacteriophages Base Sequence Chromatography, Affinity Cobra Neurotoxin Proteins DNA Primers Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Peptides Protein Binding Journal Article

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Woo-Hyeon Byeon, Bernard Weisblum. 2004-06-15. Affinity adsorbent based on combinatorial phage display peptides that bind alpha-cobratoxin.. https://doi.org/10.1016/j.jchromb.2004.03.019

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