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PubMed · 15146127

B-cell development and pre-B-1 cell plasticity in vitro.

Antonius G Rolink
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Abstract

B-lymphopoiesis in vivo is a very complex process that is influenced by cooperation between cells, cytokines, and other receptor-ligand interactions, which developmentally occur at different cellular stages. Various in vitro models have been very useful in unraveling this complex process of B-cell development. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreover, the protocols of how to grow and to test the plasticity of Pax-5-deficient pre-B-1 cells are also outlined.

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AnimalsAntigens, CDB-LymphocytesCells, CulturedDNA-Binding ProteinsFlow CytometryHematopoietic Stem CellsImmunoglobulin MLymphopoiesisMiceModels, ImmunologicalPAX5 Transcription FactorRetroviridaeStromal CellsTranscription FactorsVirus AssemblyJournal ArticleResearch Support, Non-U.S. Gov't

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Antonius G Rolink. 2004. B-cell development and pre-B-1 cell plasticity in vitro.. https://doi.org/10.1385/1-59259-796-3%3A271

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