Gene expression studies using self-fabricated parasite cDNA microarrays.

Karl F Hoffmann; Jennifer M Fitzpatrick

doi:10.1385/1-59259-793-9:219

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Gene expression studies using self-fabricated parasite cDNA microarrays.

Karl F Hoffmann·Jennifer M Fitzpatrick

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Abstract

DNA microarray platforms represent a functional genomics technology that uses structured information obtained from genomic sequencing efforts as a means to study transcriptional processes in a systematic and high-throughput manner. Specifically in this chapter, we outline the ordered processes involved in large-scale parasite gene expression studies including complementary (cDNA) microarray fabrication, total RNA isolation, cDNA labeling using fluorochromes, and DNA:DNA hybridization. Methods described herein were adapted for the study of schistosome sexual maturation and developmental biology but could be easily modified for the study of any additional parasitological system.

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Animals Base Sequence DNA Primers DNA, Complementary Gene Expression Oligonucleotide Array Sequence Analysis Parasites Polymerase Chain Reaction Journal Article

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Karl F Hoffmann, Jennifer M Fitzpatrick. 2004. Gene expression studies using self-fabricated parasite cDNA microarrays.. https://doi.org/10.1385/1-59259-793-9%3A219

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