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FISH mapping for helminth genome.

Hirohisa Hirai·Yuriko Hirai
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Abstract

Basic techniques for fluorescence in situ hybridization (FISH) mapping that have been used in genome projects on schistosomes and filariae are introduced. The chapter shows techniques specific for bacterial artificial chromosome (BAC) and yeast artificial chromosome (YAC) clones and includes experiences of chromosome preparation, DNA labeling, hybridization, microscopy, and localization of BAC clones.

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AnimalsGenomeHelminthsIn Situ Hybridization, FluorescenceJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Hirohisa Hirai, Yuriko Hirai. 2004. FISH mapping for helminth genome.. https://doi.org/10.1385/1-59259-793-9%3A379

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