Technique review: how to use RNA interference.

Ben Lehner; Andrew G Fraser; Christopher M Sanderson

doi:10.1093/bfgp/3.1.68

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Technique review: how to use RNA interference.

Ben Lehner·Andrew G Fraser·Christopher M Sanderson

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Abstract

RNA interference (RNAi) has been rapidly adopted as a general method for inhibiting gene expression in most laboratory organisms. This paper discusses how libraries of RNAi reagents are being used to perform genome-wide reverse genetic screens in both model organisms and mammalian cells. Guidelines for designing effective small interfering RNAs and appropriate controls for mammalian RNAi experiments will also be discussed.

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Animals Cells, Cultured Gene Library Genetic Techniques Humans RNA Interference RNA, Messenger RNA, Small Interfering Recombination, Genetic Journal Article Review

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Ben Lehner, Andrew G Fraser, Christopher M Sanderson. 2004. Technique review: how to use RNA interference.. https://doi.org/10.1093/bfgp%2F3.1.68

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