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Solid-phase extraction-liquid chromatographic method for the determination and pharmacokinetic studies of albiflorin and paeoniflorin in rat serum after oral administration of Si-Wu decoction.

Yuxin Sheng·Lie Li·Chuanshe Wang·Yanyan Li·Dean Guo
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Abstract

A sensitive and rapid high-performance liquid chromatography (HPLC) method with solid-phase extraction (SPE) to simultaneously determine albiflorin and paeoniflorin in rat serum was described. Serum samples were pretreated with solid-phase extraction using Extract-Clean cartridges, and the extracts were analyzed by HPLC on a reversed-phase C(18) column and a mobile phase of acetonitrile-0.03% formic acid (17:83 (v/v)) with ultraviolet detection at 230 nm. Pentoxifylline was used as the internal standard (IS). The linear ranges of the calibration curves were 29-1450 ng/ml for albiflorin and 10-2000 ng/ml for paeoniflorin. The intra- and inter-day precisions (R.S.D.) were <or=10.49% for albiflorin and <or=11.29% for paeoniflorin, respectively. Mean recovery was determined to be 89.75% for albiflorin and 85.82% for paeoniflorin. The limit of quantification was 29 ng/ml for albiflorin and 10 ng/ml for paeoniflorin, respectively. The validated method was applicable to pharmacokinetic studies of albiflorin and paeoniflorin from rat serum after oral administration of Si-Wu decoction. The pharmacokinetic study indicated that albiflorin and paeoniflorin had poor absorption and rapid elimination. This assay result was necessary for the pharmacokinetic evaluation of Si-Wu decoction.

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Administration, OralAnimalsBenzoatesBridged-Ring CompoundsChromatography, High Pressure LiquidDrugs, Chinese HerbalGlucosidesMonoterpenesRatsReproducibility of ResultsGlycosidesJournal ArticleResearch Support, Non-U.S. Gov't

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Yuxin Sheng, Lie Li, Chuanshe Wang, Yanyan Li, Dean Guo. 2004-07-05. Solid-phase extraction-liquid chromatographic method for the determination and pharmacokinetic studies of albiflorin and paeoniflorin in rat serum after oral administration of Si-Wu decoction.. https://doi.org/10.1016/j.jchromb.2004.03.041

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