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Simple, rapid and selective method using high-performance liquid chromatography for the determination of bretylium in plasma.

Y Théorêt·F Varin
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Abstract

A high-performance liquid chromatographic method with ultraviolet detection has been developed for the determination of bretylium in plasma. Following a single-step solid-phase extraction procedure, bretylium is selectively isolated and well recovered from plasma. The assay sensitivity is 0.156 micrograms/ml from 250-microliters plasma samples and its linearity was assessed up to 40 micrograms/ml. The method is accurate (101.0 +/- 5.4%) and precise (maximum coefficient of variation of 8%). It provides a simple and time-saving alternative to existing methods and is particularly suitable for pharmacokinetic studies.

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AnimalsBretylium CompoundsChromatography, High Pressure LiquidHumansSwineUltraviolet RaysJournal Article

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Y Théorêt, F Varin. 1992-03-13. Simple, rapid and selective method using high-performance liquid chromatography for the determination of bretylium in plasma.. https://doi.org/10.1016/0378-4347(92)80519-v

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