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PubMed · 15173352

High-throughput polyribosome fractionation.

Yipeng Wang·Steven Ringquist·Ann H Cho·Gaelle Rondeau·John Welsh
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Abstract

Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose gradients prepared in deep 96-well plates, the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneously in a tabletop centrifuge.

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Cell FractionationCell Line, TumorCentrifugation, Density GradientGene Expression RegulationHumansPolyribosomesProtein BiosynthesisPuromycinRNA, MessengerReverse Transcriptase Polymerase Chain ReactionUltracentrifugationJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Yipeng Wang, Steven Ringquist, Ann H Cho, Gaelle Rondeau, John Welsh. 2004-06-01. High-throughput polyribosome fractionation.. https://doi.org/10.1093/nar%2Fgnh077

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