Determining protein half-lives.

Pengbo Zhou

doi:10.1385/1-59259-816-1:067

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Determining protein half-lives.

Pengbo Zhou

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Abstract

Controlling the stability of cellular proteins is a fundamental way by which cells regulate growth, differentiation, survival, and development. Measuring the turnover rate of a protein is often the first step in assessing whether or not the function of a protein is regulated by proteolysis under specific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-established. This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein's half-life in yeast and cultured mammalian cells.

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Cycloheximide Cysteine Endopeptidases Fungal Proteins Half-Life HeLa Cells Humans Immunoblotting Isotope Labeling Methods Multienzyme Complexes Promoter Regions, Genetic Proteasome Endopeptidase Complex Protein Processing, Post-Translational Protein Synthesis Inhibitors Proteins Yeasts Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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Pengbo Zhou. 2004. Determining protein half-lives.. https://doi.org/10.1385/1-59259-816-1%3A067

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