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Techniques in protein methylation.

Jaeho Lee·Donghang Cheng·Mark T Bedford
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Abstract

Proteins can be methylated on the side-chain nitrogens of arginine and lysine residues or on carboxy-termini. Protein methylation is a way of subtly changing the primary sequence of a peptide so that it can encode more information. This common posttranslational modification is implicated in the regulation of a variety of processes including protein trafficking, transcription and protein-protein interactions. In this chapter, we will use the arginine methyltransferases to illustrate different approaches that have been developed to assess protein methylation. Both in vivo and in vitro methylation techniques are described, and the use of small molecule inhibitors of protein methylation will be demonstrated.

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Adaptor Proteins, Signal TransducingAdenosineDNA-Binding ProteinsEnzyme InhibitorsGlutathione TransferaseHeLa CellsHistonesHumansIntracellular Signaling Peptides and ProteinsMethylationMethyltransferasesPoly(A)-Binding Protein IPrecipitin TestsProtein Processing, Post-TranslationalProtein-Arginine N-MethyltransferasesRNA-Binding ProteinsRecombinant Fusion ProteinsS-AdenosylmethionineSignal TransductionSubstrate SpecificityJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Jaeho Lee, Donghang Cheng, Mark T Bedford. 2004. Techniques in protein methylation.. https://doi.org/10.1385/1-59259-816-1%3A195

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