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PubMed · 15174108

Polyethylene glycol-grafted polystyrene particles.

Fenghua Meng·Gerard H M Engbers·Jan Feijen
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Abstract

Densely pegylated particles that can serve as a model system for artificial cells were prepared by covalently grafting amino polyethylene glycol (PEG, molecular weight 3400 or 5000) onto carboxyl polystyrene particles (PS-COOH) using carbodiimide chemistry. PEG-modified particles (PS-PEG) were characterized by determination of the PEG surface concentration, zeta-potential, size, and morphology. Under optimized grafting conditions, a dense "brush-like" PEG layer was formed. A PEG surface concentration of approximately 60 pmol/cm2, corresponding with an average distance between grafted PEG chains of approximately 17 A can be realized. It was shown that grafting of PEG onto PS-COOH reduced the adsorption of proteins from human plasma (85 vol %) in phosphate-buffered saline up to 90%.

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AdsorptionAminesBiocompatible MaterialsBlood ProteinsElectric ConductivityElectrochemistryHumansHydrogen-Ion ConcentrationIndicators and ReagentsMagnetic Resonance SpectroscopyMicroscopy, Electron, ScanningMolecular WeightParticle SizePlasmaPolyethylene GlycolsPolystyrenesSurface PropertiesTrinitrobenzenesulfonic AcidJournal Article

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Fenghua Meng, Gerard H M Engbers, Jan Feijen. 2004-07-01. Polyethylene glycol-grafted polystyrene particles.. https://doi.org/10.1002/jbm.a.30056

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