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An improved thin-layer chromatography/mass spectrometry coupling using a surface sampling probe electrospray ion trap system.

Michael J Ford·Gary J Van Berkel
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Abstract

A combined surface sampling probe/electrospray emitter coupled with an ion trap mass spectrometer was used for the direct read out of unmodified reversed-phase C18 thin-layer chromatography (TLC) plates. The operation of the surface sampling electrospray ionization interface in positive and negative ionization modes was demonstrated through the direct analysis of TLC plates on which a commercial test mix comprised of four dye compounds viz., rhodamine B, fluorescein, naphthol blue black, and fast green FCF, and an extract of the caffeine-containing plant Ilex vomitoria, were spotted and developed. Acquisition of full-scan mass spectra and automated collection of MS/MS product ion spectra while scanning a development lane along the surface of a TLC plate demonstrated the advantages of using an ion trap in this combination. Details of the sampling system, benefits of analyzing a developed lane in both positive ion and negative ion modes, levels of detection while surface scanning, surface scan speed effects, and the utility of three-dimensional data display, are also discussed.

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Amido BlackChromatography, Thin LayerColoring AgentsFluoresceinIlex vomitoriaLissamine Green DyesPlant ExtractsRhodaminesSpectrometry, Mass, Electrospray IonizationJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Michael J Ford, Gary J Van Berkel. 2004. An improved thin-layer chromatography/mass spectrometry coupling using a surface sampling probe electrospray ion trap system.. https://doi.org/10.1002/rcm.1486

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