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PubMed · 15176796

2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures.

Thazha P Prakash·Ask Püschl·Elena Lesnik·Venkatraman Mohan·Valentina Tereshko·Martin Egli·Muthiah Manoharan
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Abstract

[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.

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DNADNA, ComplementaryGuanidineModels, MolecularNucleic Acid ConformationOligonucleotidesRNARNA, ComplementaryTime FactorsJournal Article

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Thazha P Prakash, Ask Püschl, Elena Lesnik, Venkatraman Mohan, Valentina Tereshko, Martin Egli, Muthiah Manoharan. 2004-06-10. 2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures.. https://doi.org/10.1021/ol049470e

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