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PubMed · 15179534

Silica-nanoparticle-based interface for the enhanced immobilization and sequence-specific detection of DNA.

D Zhang·Y Chen·H-Y Chen·X H Xia
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Abstract

A biocompatible and uniform interface based on silica nanoparticles derivatized with amino groups has been constructed for the effective immobilization and sensitive sequence-specific detection of calf thymus DNA. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) results showed that a monolayer of silica nanoparticles can be formed on a gold electrode under our experimental conditions using cysteine self-assembly monolayer as binder medium. Electrochemical impedance spectroscopy and X-ray photoelectron spectroscopy (XPS) verified the successful immobilization of DNA on silica-nanoparticle-modified gold electrodes. Quantitative results demonstrated that enhanced immobilization of single-strand DNA (ss-DNA) up to 1.6 x 10(-8) mol cm(-2) could be achieved owing to the larger surface area and the special properties of silica nanoparticles. In addition, hybridization experiments demonstrated that the immobilized ss-DNA on silica nanoparticles could specifically interact with complementary DNA in solutions.

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AnimalsCattleDNADNA, Single-StrandedGoldImmobilizationNanostructuresSensitivity and SpecificitySequence Analysis, DNASilicon DioxideSurface PropertiesJournal ArticleResearch Support, Non-U.S. Gov't

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D Zhang, Y Chen, H-Y Chen, X H Xia. 2004-06-04. Silica-nanoparticle-based interface for the enhanced immobilization and sequence-specific detection of DNA.. https://doi.org/10.1007/s00216-004-2653-7

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